Recombinant Human DLL3 protein, His-tagged

• Cat.No.: DLL3-5654H
• Product Overview: Recombinant Human DLL3 protein(Q9NYJ7-1)(Ala27-Arg490), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ala27-Arg490
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human DLL3, His Tag at 1 μg/mL (100 μl/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 1.2 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: DLL3 delta-like 3 (Drosophila) [ Homo sapiens ]
• Official Symbol: DLL3
• Synonyms: DLL3; delta-like 3 (Drosophila); delta (Drosophila) like 3; delta-like protein 3; SCDO1; delta3; drosophila Delta homolog 3
• Gene ID: 10683
• mRNA Refseq: NM_016941
• Protein Refseq: NP_058637
• MIM: 602768
• UniProt ID: Q9NYJ7