Recombinant Human DKK3 protein, His-tagged

• Cat.No.: DKK3-685H
• Product Overview: Recombinant Human DKK3 (Pro23-Ile350) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Pro23-Ile350
• Description: Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.
• Predicted N Terminal: Pro23
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 37.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Publications:
  Use of tumor suppressor genes of naked mole rats for human cancer treatment (2023)

Gene Information

• Gene Name: DKK3
• Official Symbol: DKK3
• Synonyms: DKK3; dickkopf 3 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 3; dickkopf-related protein 3; regulated in glioma; REIC; RIG; dkk-3; hDkk-3; dickkopf-3; RIG-like 5-6; RIG-like 7-1; dickkopf homolog 3
• Gene ID: 27122
• mRNA Refseq: NM_001018057
• Protein Refseq: NP_001018067
• MIM: 605416
• UniProt ID: Q9UBP4