Recombinant Human DKK1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: DKK1-490HB
• Product Overview: Biotinylated Recombinant Human Dickkopf-related protein 1 is produced by our Mammalian expression system and the target gene encoding Thr32-His266 is expressed with a Avi, 6His tag at the C-terminus.

Specification

• Description: Dickkopf-related protein 1(DKK-1), is a member of the dickkopf family. DKK1 secreted proteins with two cysteine-rich domains separated by a linker region. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
• Source: HEK293
• Species: Human
• Tag: His&Avi
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Molecular Mass: 35-50kDa, reducing conditions.
• Protein length: Thr32-His266
• Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: DKK1 dickkopf WNT signaling pathway inhibitor 1 [ Homo sapiens (human) ]
• Official Symbol: DKK1
• Synonyms: Dickkopf-related protein 1; Dickkopf-1; Dkk-1
• Gene ID: 22943
• mRNA Refseq: NM_012242.4
• Protein Refseq: NP_036374.1
• MIM: 605189
• UniProt ID: O94907