Recombinant Human Deoxyhypusine Synthase, His-tagged

• Cat.No.: DHPS-568H
• Product Overview: Recombinant Human DHPS protein was expressed inE.coliwith His tag and purified by conventional chromatography techniques. MW=43.1 kDa (389aa).

Specification

• Cat. No.: DHPS-568H
• Description: DHPS, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. This protein catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue.
• Source: E.coli.
• Sequences: MGSSHHHHHH SSGLVPRGSH MEGSLEREAP AGALAAVLKH SSTLPPESTQ VRGYDFNRGV NYRALLEAFG TTGFQATNFG RAVQQVNAMI EKKLEPLSQD EDQHADLTQS RRPLTSCTIF LGYTSNLISS GIRETIRYLV QHNMVDVLVT TAGGVEEDLI KCLAPTYLGE FSLRGKELRE NGINRIGNLL VPNENYCKFE DWLMPILDQM VMEQNTEGVK WTPSKMIARL GKEINNPESV YYWAQKNHIP VFSPALTDGS LGDMIFFHSY KNPGLVLDIV EDLRLINTQA IFAKCTGMII LGGGVVKHHI ANANLMRNGA DYAVYINTAQ EFDGSDSGAR PDEAVSWGKI RVDAQPVKVY ADASLVFPLL VAETFAQKMD AFMHEKNED.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DHPS deoxyhypusine synthase [ Homo sapiens ]
• Synonyms: DHPS; deoxyhypusine synthase; MIG13; migration-inducing gene 13; DHS; EC 2.5.1.46
• Gene ID: 1725
• mRNA Refseq: NM_001930
• Protein Refseq: NP_001921
• MIM: 600944
• UniProt ID: P49366
• Chromosome Location: 19p13.2-p13.1
• Pathway: Metabolism of proteins
• Function: deoxyhypusine synthase activity; protein binding; transferase activity

Reviews

12/26/2020

Their expert team has been instrumental in addressing any challenges or concerns I have encountered during my experiments.

02/14/2019

Their prompt and comprehensive assistance has proven invaluable in overcoming obstacles and optimizing my experimental protocols.

02/05/2019

The DHPS protein boasts remarkable stability, allowing for extended storage without compromising its integrity or performance.

Q&As

Are there any resistance mechanisms against sulfonamides targeting DHPS?

Yes, bacterial resistance to sulfonamides can arise due to mutations in DHPS that reduce the drug's binding affinity.

Can DHPS mutations affect the effectiveness of sulfonamides in treating bacterial infections?

Yes, mutations in DHPS can lead to reduced effectiveness of sulfonamides, contributing to antibiotic resistance.

Are there any other clinical implications of DHPS beyond antibiotic treatment?

DHPS mutations have been associated with resistance to sulfadoxine-pyrimethamine, a drug used to prevent and treat malaria.

In what clinical conditions are sulfonamides commonly used?

Sulfonamides are used to treat urinary tract infections, respiratory tract infections, and other bacterial infections.

Can DHPS be a target for drug development beyond antibiotics?

DHPS has potential as a target for developing new antiparasitic drugs due to its role in folate biosynthesis in parasites such as Plasmodium.