Recombinant Human Dehydrogenase/Reductase (SDR family) Member 4, His-tagged

• Cat.No.: DHRS4-1326H
• Product Overview: Recombinant human DHRS4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: DHRS4, also known as dehydrogenase/reductase SDR family member 4, belongs to the short-chain dehydrogenases/reductases (SDR) family. DHRS4 reduces all trans retinal and 9 cis retinal. This protein can also catalyze the oxidation of all trans retinol with NADP as cofactor, but with much lower efficiency. Also, DHRS4 reduces alkyl phenyl ketones and alpha dicarbonyl compounds with aromatic rings, such as pyrimidine 4 aldehyde, 3 benzoylpyridine, 4 benzoylpyridine, menadione and 4 hexanoylpyridine.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol.
• Molecular Weight: 32.1 kDa (302 aa), confirmed by MALDI-TOF
• Purity: > 85 % by SDS-PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMHKAGL LGLCARAWNS VRMASSGMTR RDPLANKVAL VTASTDGIGF AIARRLAQDG AHVVVSSRKQ QNVDQAVATL QGEGLSVTGT VCHVGKAEDR ERLVATAVKL HGGIDILVSN AAVNPFFGSI MDVTEEVWDK TLDINVKAPA LMTKAVVPEM EKRGGGSVVI VSSIAAFSPS PGFSPYNVSK TALLGLTKTL AIELAPRNIR VNCLAPGLIK TSFSRMLWMD KEKEESMKET LRIRRLGEPE DCAGIVSFLC SEDASYITGE TVVVGGGTPS RL
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Metabolic pathways; Peroxisome; Retinol metabolism

Gene Information

• Gene Name: DHRS4 dehydrogenase/reductase (SDR family) member 4 [ Homo sapiens ]
• Official Symbol: DHRS4
• Synonyms: DHRS4; dehydrogenase/reductase (SDR family) member 4; CR; NRDR; PHCR; PSCD; SDR-SRL; SDR25C1; SDR25C2; FLJ11008; SCAD-SRL; dehydrogenase/reductase SDR family member 4; OTTHUMP00000164690; NADP-dependent retinol dehydrogenase; peroxisomal short-chain alcohol dehydrogenase; NADPH-dependent retinol dehydrogenase/reductase; short-chain dehydrogenase/reductase family member 4; dehydrogenase/reductase (SDR family) member 4 like 2A; short chain dehydrogenase/reductase family 25C, member 1; short chain dehydrogenase/reductase family 25C, member 2; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; NP_066284.2; EC 1.1.1.184
• Gene ID: 10901
• mRNA Refseq: NM_021004
• Protein Refseq: NP_066284
• MIM: 611596
• UniProt ID: Q9BTZ2
• Chromosome Location: 14q11.2
• Function: binding; carbonyl reductase (NADPH) activity; oxidoreductase activity