Recombinant Human DEFA4 protein, His & GST-tagged

• Cat.No.: DEFA4-1143H
• Product Overview: Recombinant Human DEFA4 aa. (Gln23~Asp97 (Accession # P12838) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from other genes of this family by an extra 83-base segment that is apparently the result of a recent duplication within the coding region. The protein encoded by this gene, defensin, alpha 4, is found in the neutrophils; it exhibits corticostatic activity and inhibits corticotropin stimulated corticosterone production.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 40.6kDa.
• Protein length: Gln23~Asp97
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 6.4.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: DEFA4 defensin alpha 4 [ Homo sapiens (human) ]
• Official Symbol: DEFA4
• Synonyms: DEFA4; defensin alpha 4; HP4; DEF4; HP-4; HNP-4; neutrophil defensin 4; corticostatin; defensin, alpha 4, corticostatin; defensin, alpha 4, preproprotein
• Gene ID: 1669
• mRNA Refseq: NM_001925.2
• Protein Refseq: NP_001916.1
• UniProt ID: P12838