Recombinant Human DBR1, His-tagged

• Cat.No.: DBR1-2340H
• Product Overview: Recombinant human DBR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: DBR1 is an RNA lariat debranching enzyme that hydrolyzes 2"-5" prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.
• Molecular Mass: 38 kDa (Molecular size on SDS-PAGE will appear higher)
• Protein length: 340aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDDLINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE EDEDSIVSAH SGMNTPSVEP SDQASEFSAS FSDVRILPGSMIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDDDDDAA
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: DBR1 debranching enzyme homolog 1 (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: DBR1
• Synonyms: DBR1; debranching enzyme homolog 1 (S. cerevisiae); debranching enzyme (S. Cerevisiae) homolog 1; lariat debranching enzyme; RNA lariat debranching enzyme
• Gene ID: 51163
• mRNA Refseq: NM_016216
• Protein Refseq: NP_057300
• MIM: 607024
• UniProt ID: Q9UK59
• Chromosome Location: 3q22.3
• Function: RNA lariat debranching enzyme activity; hydrolase activity, acting on ester bonds; metal ion binding