Recombinant Human DAZL Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DAZL-5248H
• Product Overview: DAZL MS Standard C13 and N15-labeled recombinant protein (NP_001342) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 33 kDa
• AA Sequence: MSTANPETPNSTISREASTQSSSAATSQGYILPEGKIMPNTVFVGGIDVRMDETEIRSFFARYGSVKEVKIITDRTGVSKGYGFVSFFNDVDVQKIVESQINFHGKKLKLGPAIRKQNLCAYHVQPRPLVFNHPPPPQFQNVWTNPNTETYMQPTTTMNPITQYVQAYPTYPNSPVQVITGYQLPVYNYQMPPQWPVGEQRSYVVPPAYSAVNYHCNEVDPGAEVVPNECSVHEATPPSGNGPQKKSVDRSIQTVVSCLFNPENRLRNSVVTQDDYFKDKRVHHFRRSRAMLKSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DAZL deleted in azoospermia-like [ Homo sapiens (human) ]
• Official Symbol: DAZL
• Synonyms: DAZL; deleted in azoospermia-like; DAZLA; DAZH; DAZL1; MGC26406; SPGYLA; DAZ homolog; DAZ-like autosomal; SPGY-like-autosomal; deleted in azoospermia-like 1; germline specific RNA binding protein; spermatogenesis gene on the Y-like autosomal
• Gene ID: 1618
• mRNA Refseq: NM_001351
• Protein Refseq: NP_001342
• MIM: 601486
• UniProt ID: Q92904