Recombinant Human/Cynomolgus/Rhesus macaque ROR1 protein, hFc-tagged

• Cat.No.: ROR1-183H
• Product Overview: Recombinant Human/Cynomolgus/Rhesus macaque ROR1 protein(Q01973-1)(Gln30-Glu403), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human/Cynomolgus/Rhesus macaque
• Tag: C-hFc
• Protein length: Gln30-Glu403
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human/Cynomolgus/Rhesus macaque ROR1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-ROR1 Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 68.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.