Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, His-Avi-tagged

• Cat.No.: CD28-5744H
• Product Overview: Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein(P10747-1)(Asn19-Pro152), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human/Cynomolgus/Rhesus macaque
• Tag: N-His-Avi
• Protein length: Asn19-Pro152
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 18.4 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.