Recombinant Human Cyclin-Dependent Kinase 9, GST-tagged, Active

• Cat.No.: CDK9-280H
• Product Overview: Recombinant human full-length CDK9 and CyclinK were co-expressed by baculovirus inSf9insect cells using an N-terminal GST tag on both proteins. MW=68 kDa and CyclinK~67 kDa.

Specification

• Cat. No.: CDK9-280H
• Description: CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is closely related to cdc28 and cdc2 and is an important regulator of the cell cycle. CDK9 is a component of the multiprotein complex TAK/P-TEFβ. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the HIV-1 Tat protein which suggested a possible involvement of this protein in AIDS.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: CDK9 cyclin-dependent kinase 9 [ Homo sapiens ]
• Synonyms: CDK9; cyclin-dependent kinase 9; TAK; C-2k; CTK1; CDC2L4; PITALRE; cyclin-dependent kinase 9 (CDC2-related kinase);Cell division protein kinase 9;EC2.7.11.22;EC2.7.11.23; Cyclin-dependent kinase 9; Serine/threonine-protein kinase; Cell division cycle 2-like protein kinase 4
• Gene ID: 1025
• mRNA Refseq: NM_001261
• Protein Refseq: NP_001252
• UniProt ID: P50750
• Chromosome Location: 9q34.1
• MIM: 603251
• Pathway: Gene Expression; HIV Infection; Transcription
• Function: ATP binding; DNA binding; RNA polymerase II carboxy-terminal domain kinase activity; cyclin-dependent protein kinase activity; nucleotide binding; protein binding; snRNA binding; transferase activity