Recombinant Human cyclic GMP-AMP synthase Protein, His tagged

• Cat.No.: CGAS-201H
• Product Overview: Recombinant human CGAS protein (Q8N884-1) (Asp 157-Phe 522) with a His tag at the C-terminus was expressed in E. coli.

Specification

• Description: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (2',3'-cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G (2',5')pA (3',5')p]. Acts as a key DNA sensor: directly binds double-stranded DNA (dsDNA), inducing the formation of liquid-like droplets in which CGAS is activated, leading to synthesis of 2',3'-cGAMP, a second messenger that binds to and activates STING1, thereby triggering type-I interferon production. Acts as a key foreign DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses.
• Source: E. coli
• Species: Human
• Tag: C-His
• Protein length: 157-522aa
• Molecular Mass: 44.4 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: CGAS cyclic GMP-AMP synthase [ Homo sapiens (human) ]
• Official Symbol: CGAS
• Synonyms: CGAS; cyclic GMP-AMP synthase; D4; MB21D1; h-cGAS; C6orf150; cyclic GMP-AMP synthase; 2'3'-cGAMP synthase; DANGER family member 4; Mab-21 domain containing 1; cGAMP synthase; mab-21 domain-containing protein 1; protein MB21D1; EC 2.7.7.86
• Gene ID: 115004
• mRNA Refseq: NM_138441.3
• Protein Refseq: NP_612450.2
• MIM: 613973
• UniProt ID: Q8N884