Recombinant Human CTLA4, Fc Chimera

• Cat.No.: CTLA4-1913H
• Product Overview: Recombinant Human CTLA-4 produced inE.Coliis a homodimeric, non-glycosylated polypeptide chain containing a total molecular mass of 80 kDa. Each subunit (40kDa) is fused to a polypeptide linker to the Fc portion of Human IgG1. rHuCTLA-4 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Fc
• Description: CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Ala-Met-His-Val.
• Form: Purified.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC.
• Dimers & Aggregates: <1% as determined by silver stained SDS-PAGE gel analysis.
• Endotoxin: < 0.1 ng/µg (IEU/µg) of rHuCTLA-4.
• Protein Content: Protein quantitation was carried out by two independent methods: 1) UV spectroscopy at 280 nm. 2) Analysis by RP-HPLC, using a standard solution of CTLA-4 as a Reference Standard.
• Application: 1) Molecular standard (Western, ELISA) in studying secreted BMP-4. 2) Preparing antibodies for BMP-4 monomer. 3) Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.
• Biological Activity: rHuCTLA-4 is fully biologically active when compared to standards.
• Storage: -20°C, avoid freezing and thawing cycles after reconstitution.

Gene Information

• Gene Name: CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens ]
• Synonyms: CTLA4; cytotoxic T-lymphocyte- associated protein 4; CD; CD28; CD152; CD152; CELIAC3; CTLA-4; celiac disease 3; Cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; ICOS; GSE; IDDM12; IDDM12; CTLA-4; OTTHUMP00000163781
• Gene ID: 1493
• mRNA Refseq: NM_001037631
• Protein Refseq: NP_001032720
• MIM: 123890
• UniProt ID: P16410
• Chromosome Location: 2q33
• Pathway: Autoimmune thyroid disease ; Cell adhesion molecules (CAMs); T cell receptor signaling pathway
• Function: Protein binding