Recombinant Human CPE protein, His & GST-tagged

• Cat.No.: CPE-1438H
• Product Overview: Recombinant Human CPE aa. (Ile151~Gly317 (Accession # P16870) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Ile151~Gly317
• Description: This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 50.8kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: CPE carboxypeptidase E [ Homo sapiens (human) ]
• Official Symbol: CPE
• Synonyms: CPE; carboxypeptidase E; CPH; carboxypeptidase H; cobalt-stimulated chromaffin granule carboxypeptidase; enkephalin convertase; insulin granule-associated carboxypeptidase; prohormone-processing carboxypeptidase
• Gene ID: 1363
• mRNA Refseq: NM_001873.3
• Protein Refseq: NP_001864.1
• UniProt ID: P16870