Recombinant Human core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1, His-tagged

• Cat.No.: NAA30-227H
• Product Overview: NAA30, 30-363aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 30-363 a.a.
• Description: C1GALT1 generates the common core 1 O-glycan structure, Gal-beta-1-3GalNAc-R, by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended mucin-type O-glycans on cell surface and secreted glycoproteins. Studies in mice suggest that this gene plays a key role in thrombopoiesis and kidney homeostasis. Recombinant human C1GALT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 41.4 kDa (357aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSLLGEKVD TQPNVLHNDP HARHSDDNGQ NHLEGQMNFN ADSSQHKDEN TDIAENLYQK VRILCWVMTG PQNLEKKAKH VKATWAQRCN KVLFMSSEEN KDFPAVGLKT KEGRDQLYWK TIKAFQYVHE HYLEDADWFL KADDDTYVIL DNLRWLLSKY DPEEPIYFGR RFKPYVKQGY MSGGAGYVLS KEALKRFVDA FKTDKCTHSS SIEDLALGRC MEIMNVEAGD SRDTIGKETF HPFVPEHHLI KGYLPRTFWY WNYNYYPPVE GPGCCSDLAV SFHYVDSTTM YELEYLVYHL RPYGYLYRYQ PTLPERILKE ISQANKNEDT KVKLGNP
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bardford assay)

Gene Information

• Gene Name: NAA30 N(alpha)-acetyltransferase 30, NatC catalytic subunit [ Homo sapiens ]
• Official Symbol: NAA30
• Synonyms: NAA30; N(alpha)-acetyltransferase 30, NatC catalytic subunit; C14orf35, chromosome 14 open reading frame 35 , N acetyltransferase 12 , N acetyltransferase 12 (GCN5 related, putative) , NAT12; N-alpha-acetyltransferase 30; FLJ35355; MAK3; Mak3p; natC catalytic subunit; putative N-acetyltransferase; N-acetyltransferase MAK3 homolog; N-acetyltransferase 12 (GCN5-related, putative); N-alpha-acetyltransferase 30, NatC catalytic subunit; NAT12; C14orf35; MGC141884; MGC141885
• Gene ID: 122830
• mRNA Refseq: NM_001011713
• Protein Refseq: NP_001011713
• UniProt ID: Q147X3
• Chromosome Location: 14q22.2
• Function: N-acetyltransferase activity; peptide alpha-N-acetyltransferase activity; transferase activity, transferring acyl groups