Recombinant Human collapsin response mediator protein 1 protein, His tagged

• Cat.No.: CRMP1-12HFL
• Product Overview: Recombinant human CRMP1 (1-572 aa), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-572 aa
• Description: This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants.
• Tag: N-His
• Form: Liquid
• Molecular Mass: 64.8 kDa (597aa)
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMGSEFMSYQGKKSIPHITSDRLLIKGGRIINDDQSLYADVYLEDGLIKQIGENLIVPGGVKTIEANGRMVIPGGIDVNTYLQKPSQGMTAADDFFQGTRAALVGGTTMIIDHVVPEPGSSLLTSFEKWHEAADTKSCCDYSLHVDITSWYDGVREELEVLVQDKGVNSFQVYMAYKDVYQMSDSQLYEAFTFLKGLGAVILVHAENGDLIAQEQKRILEMGITGPEGHALSRPEELEAEAVFRAITIAGRINCPVYITKVMSKSAADIIALARKKGPLVFGEPIAASLGTDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYLTSLLACGDLQVTGSGHCPYSTAQKAVGKDNFTLIPEGVNGIEERMTVVWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADVVIWDPDKLKTITAKSHKSAVEYNIFEGMECHGSPLVVISQGKIVFEDGNINVNKGMGRFIPRKAFPEHLYQRVKIRNKVFGLQGVSRGMYDGPVYEVPATPKYATPAPSAKSSPSKHQPPPIRNLHQSNFSLSGAQIDDNNPRRTGHRIVAPPGGRSNITSLG
• Endotoxin: < 1 EU/μg by LAL
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT, 0.2mM PMSF
• Concentration: 1 mg/mL (determined by Bradford assay)
• Reference: 1. Shih J.-Y. et al. (2001) J. Natl. Cancer Inst. 93:1392-1400.
  2. Chen T.C. et al. (2009) J. Proteome Res. 8:4943-4953.

Gene Information

• Gene Name: CRMP1 collapsin response mediator protein 1 [ Homo sapiens (human) ]
• Official Symbol: CRMP1
• Synonyms: CRMP1; collapsin response mediator protein 1; dihydropyrimidinase-related protein 1; DPYSL1; DRP 1; dihydropyrimidinase-like 1; unc-33-like phosphoprotein 3; dihydropyrimidinase related protein-1; DRP1; DRP-1; CRMP-1; ULIP-3
• Gene ID: 1400
• mRNA Refseq: NM_001313
• Protein Refseq: NP_001304
• MIM: 602462
• UniProt ID: Q14194