Recombinant Human COL8A1, His-tagged

Cat.No. : COL8A1-140H
Product Overview : Recombinant Human COL8A1(Pro575~Pro743), fused with with N-terminal His-Tag, was expressed in E.coli.
Availability March 14, 2025
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Species : Human
Source : E.coli
Tag : His
Protein Length : Pro575~Pro743
Description : This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed.
Form : Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Molecular Mass : 20.2kDa
AA Sequence : The target protein is fused with N-terminal His-Tag, its sequence is listed below.MGHHHHHHSGSEF-PPTPPP QGEYLPDMGL GIDGVKPPHA YGAKKGKNGG PAYEMPAFTA ELTAPFPPVG APVKFNKLLY NGRQNYNPQT GIFTCEVPGV YYFAYHVHCK GGNVWVALFK NNEPVMYTYD EYKKGFLDQA SGSAVLLLRP GDRVFLQMPSEQAAGLYAGQ YVHSSFSGYL LYP
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37ºC for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name COL8A1 collagen, type VIII, alpha 1 [ Homo sapiens (human) ]
Official Symbol COL8A1
Synonyms COL8A1; collagen, type VIII, alpha 1; C3orf7, chromosome 3 open reading frame 7; collagen alpha-1(VIII) chain; MGC9568; endothelial collagen; collagen VIII, alpha-1 polypeptide; cell proliferation-inducing protein 41; smooth muscle cell-expressed and macrophage conditioned medium-induced protein smag-64; C3orf7
Gene ID 1295
mRNA Refseq NM_001850
Protein Refseq NP_001841
MIM 120251
UniProt ID P27658
Chromosome Location 3q12.3
Pathway Assembly of collagen fibrils and other multimeric structures; Collagen biosynthesis and modifying enzymes; Collagen formation; Extracellular matrix organization
Function serine-type endopeptidase activity

Not For Human Consumption!

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