Recombinant Human CNDP2 protein(Met1-Asp475), His-tagged

• Cat.No.: CNDP2-1131H
• Product Overview: Recombinant Human CNDP2 (CAC69883.1) (Met 1-Asp 475) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-475 a.a.
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human CNDP2 consists of 485 amino acids and predicts a molecular mass of 54.2 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CNDP2 CNDP dipeptidase 2 (metallopeptidase M20 family) [ Homo sapiens ]
• Official Symbol: CNDP2
• Synonyms: CNDP2; CNDP dipeptidase 2 (metallopeptidase M20 family); PEPA, peptidase A; cytosolic non-specific dipeptidase; CN2; CPGL; cytosolic nonspecific dipeptidase (EC 3.4.13.18); FLJ10830; HsT2298; peptidase A; cytosolic nonspecific dipeptidase; glutamate carboxypeptidase-like protein 1; PEPA; FLJ35544
• Gene ID: 55748
• mRNA Refseq: NM_001168499
• Protein Refseq: NP_001161971
• MIM: 169800
• UniProt ID: Q96KP4

Case Study

Case 1: Zhang LQ, et al. Technol Cancer Res Treat. 2019

This study looks at how cytosolic nonspecific dipeptidase 2 (CND2) affects ovarian cancer. High CND2 was found in cancer tissues. Tests showed CND2 helps cancer cells grow and spread. In mouse experiments, altering CND2 levels changed tumor growth and impacted certain proteins.

Fig1. Western blot detection of CNDP2 knockdown efficiency by shRNA in SKOV3 and OVCAR3 cells or overexpression efficiency by CNDP2 expression vector in A2780 and ISEO80.

Fig2. Cytosolic nonspecific dipeptidase 2 (CNDP2) positive regulates the activation of PI3K/AKT pathway.

Case 2: Zhang Z, et al. Mol Med. 2014

Evidence points to CNDP2 having a bigger role in cancer than just being an enzyme. In gastric cancer, CNDP2 is often lower. Adding CNDP2 slowed cell growth, caused cell death, and stopped tumor growth in tests. It boosted p38 and JNK, but losing CNDP2 increased ERK activity. This signals CNDP2 helps suppress tumors by activating the MAPK pathway.

Fig1. CNDP2 protein levels were analyzed in gastric adenocarcinoma tissues with different differentiation degree.

Fig2. The effects of CNDP2 on protein markers of MAPK pathways were assessed by Western blot in tumor xenograft tissues.

Application

Recombinant CNDP2 protein is gaining attention for its potential uses, particularly in cancer research. It's being explored for its tumor-suppressing abilities, which might help slow or halt tumor growth, making it a key player in developing new cancer treatments. Research shows that CNDP2 can influence important cell signaling pathways, such as MAPK, which plays a role in cell division and growth. By delving into CNDP2's functions, scientists aim to create drugs that replicate its effects, offering new treatment avenues for cancer patients. Outside of cancer, recombinant CNDP2 may have applications in studying metabolism. Since it's an enzyme that breaks down dipeptides, it could be instrumental in understanding and potentially treating metabolic disorders. As recombinant proteins can be produced in bulk, CNDP2 might also become a valuable research tool for investigating protein interactions and cellular mechanics, benefiting fields beyond medicine like agriculture or biotechnology.

Fig1. Potential functions of CNDP2. (Moizle Grace Castro Ocariza, 2024)