Recombinant Human CHIT1 protein, His & T7-tagged

• Cat.No.: CHIT1-1672H
• Product Overview: Recombinant Human CHIT1 aa. (Ala193~Asn466 (Accession # Q13231)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Ala193~Asn466
• Description: Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.7kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 6.4

Gene Information

• Gene Name: CHIT1 chitinase 1 [ Homo sapiens (human) ]
• Official Symbol: CHIT1
• Synonyms: CHIT1; chitinase 1; CHI3; CHIT; CHITD; chitotriosidase-1; chitinase 1 (chitotriosidase); plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase
• Gene ID: 1118
• mRNA Refseq: NM_001256125.1
• Protein Refseq: NP_001243054.2
• UniProt ID: Q13231