Recombinant Human CHGA Protein, Full Length, C-His tagged

• Cat.No.: CHGA-20HFL
• Product Overview: Recombinant human CHGA (Full Length), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect cells
• Tag: His
• Protein Length: Full Length
• Description: The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene.
• Form: Liquid
• Molecular Mass: 50 kDa (448aa)
• AA Sequence: LPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 85% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 0.1mM PMSF
• GeneID: 1113
• References: 1. Nikoopour E., et al. (2011) J Immunol. 186:3831-3835.
  2. Stadinski BD., et al. (2010) Nat Immunol. 11:225-231.

Gene Information

• Gene Name: CHGA chromogranin A [ Homo sapiens (human) ]
• Official Symbol: CHGA
• Synonyms: CHGA; chromogranin A; CGA; PHE5; PHES; chromogranin-A; SP-I; betagranin (N-terminal fragment of chromogranin A); catestatin; chromofungin; parathyroid secretory protein 1; pituitary secretory protein I
• mRNA Refseq: NM_001275
• Protein Refseq: NP_001266
• MIM: 118910
• UniProt ID: P10645