Recombinant Human Charged Multivesicular Body Protein 1B, His-tagged

• Cat.No.: CHMP1B-6794H
• Product Overview: Recombinant humanCHMP1B protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CHMP1B, also knownas charged multivesicular body protein 1b, is probable peripherallyassociated component of the endosomal sorting required for transport complexIII (ESCRT-III) which is involved in multivesicular bodies (MVBs) formationand sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminalvesicles (ILVs) that are generated by invagination and scission from thelimiting membrane of the endosome and mostly are delivered to lysosomesenabling degradation of membrane proteins, such as stimulated growth factorreceptors, lysosomal enzymes and lipids
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Molecular Weight: 24.7 kDa (223aa),confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• Purity: > 85% by SDS -PAGE
• Concentration: 0.25mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMSNMEK HLFNLKFAAK ELSRSAKKCD KEEKAEKAKI KKAIQKGNME VARIHAENAIRQKNQAVNFL RMSARVDAVA ARVQTAVTMG KVTKSMAGVV KSMDATLKTM NLEKISALMD KFEHQFETLDVQTQQMEDTM SSTTTLTTPQ NQVDMLLQEM ADEAGLDLNM ELPQGQTGSV GTSVASAEQD ELSQRLARLRDQV
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CHMP1Bcharged multivesicular body protein 1B [ Homo sapiens ]
• Official Symbol: CHMP1B
• Synonyms: CHMP1B; charged multivesicular bodyprotein 1B; chromatin modifying protein 1B; charged multivesicular bodyprotein 1b; C18orf2; CHMP1.5; Vps46B; 2810405I11Rik; Charged multivesicularbody protein 1b; CHM1B_HUMAN; CHMP1.5; CHMP1b; chmp1b; Chromatin-modifyingprotein 1b; hVps46-2; Vacuolar protein sorting-associated protein 46-2;Vps46-2; vacuolar protein sorting 46-2; chromatin-modifying protein 1b;vacuolar protein sorting-associated protein 46-2; C10orf2; Vps46-2; C18-ORF2;hVps46-2
• Gene ID: 57132
• mRNA Refseq: NM_020412
• Protein Refseq: NP_065145
• MIM: 606486
• Uniprot ID: Q7LBR1
• Chromosome Location: 18p11.21
• Pathway: ESCRT-III complex,organism-specific biosystem; Endocytosis, organism-specific biosystem;Endocytosis, conserved biosystem
• Function: protein binding; protein domainspecific binding