Recombinant Human CGA & FSHB Heterotrimer Protein (Met1-Ser116 & Met1-Glu129), HlgG1 Fc-tagged

• Cat.No.: CF-1041H
• Product Overview: Recombinant Human CGA & FSHB Heterotrimer Protein (NP_000726.1)(Met1-Ser116) & (NP_000501.1)(Met1-Glu129) was produced by HEK293 Cells expression system. The CGA protein was expressed, constructed the plasmid 1. The FSHB protein was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CGA&FSHB heterodimer was purified.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• ProteinLength: Met1-Ser116 & Met1-Glu129
• Predicted N Terminal: Ala 25 & Asn 19
• Form: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CGA&FSHB consists of 450(92+358) amino acids and predicts a molecular mass of 49.7(10.2 + 39.5) kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > (83.5+13.8) % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: CGA glycoprotein hormones, alpha polypeptide [ Homo sapiens (human) ] & FSHB follicle stimulating hormone subunit beta [ Homo sapiens (human) ]
• Official Symbol: CGA & FSHB
• Synonyms: HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA & HH24
• Gene ID: 1081 & 2488
• mRNA Refseq: NM_000735 & NM_000510
• Protein Refseq: NP_000726 & NP_000501
• UniProt ID: P01215 & P01225