Recombinant Human Cell Division Cycle 25 Homolog A (S. pombe), GST-tagged

• Cat.No.: CDC25A-1577H
• Product Overview: Full-length recombinant human CDC25A was expressed in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: CDC25A (also known as cell division cycle 25 homolog A) is a member of the CDC25 family of phosphatases that are required for progression from G1 to the S phase of the cell cycle. CDC25A can activate the cyclin-dependent kinase CDC2 (also known as CDK1) by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A overexpression is detected in human cancers and this may contribute to the tumorigenesis process. CDC25A is degraded by moderate heat shock and this degradation is protected by HSP90.
• Applications: Phosphatase Assay; Western Blot
• Molecular Weight: 94 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Specific Activity: 31 nmol/min/mg
• Purity: > 80 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Activation of ATR in response to replication stress; Cell Cycle Checkpoints; Cell cycle; Cyclin A/B1 associated events during G2/M transition; Cyclin B2 mediated events; Cyclin E associated events during G1/S transition; E2F transcription factor network; E2F-enabled inhibition of pre-replication complex formation; G1 to S cell cycle control; Integrated Breast Cancer Pathway; Cell Cycle, Mitotic; Progesterone-mediated oocyte maturation; TGF-beta Receptor Signaling Pathway; miRNAs involved in DDRCell Cycle Checkpoints; Cell Cycle, Mitotic

Gene Information

• Gene Name: CDC25A cell division cycle 25 homolog A (S. pombe) [ Homo sapiens ]
• Official Symbol: CDC25A
• Synonyms: CDC25A; cell division cycle 25 homolog A (S. pombe); CDC25A2; cell division cycle 25A; M-phase inducer phosphatase 1; dual specificity phosphatase CDC25A; EC 3.1.3.48; Dual specificity phosphatase Cdc25A; cell division cycle 25 homolog A (S. cerevisiae)
• Gene ID: 993
• mRNA Refseq: NM_001789
• Protein Refseq: NP_001780
• MIM: 116947
• UniProt ID: P30304
• Chromosome Location: 3p21
• Function: hydrolase activity; protein binding; protein tyrosine phosphatase activity