Recombinant Human CDK9 protein, His & T7-tagged

Cat.No. : CDK9-2705H
Product Overview : Recombinant Human CDK9 aa. (Asp104~Phe315 (Accession # P50750)) fused with N-terminal His & T7 tag was produced in E. coli cells.
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Species : Human
Source : E.coli
Tag : His&T7
Protein Length : Asp104~Phe315
Description : The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 28.3kDa
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 7.8.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name CDK9 cyclin dependent kinase 9 [ Homo sapiens (human) ]
Official Symbol CDK9
Synonyms TAK; C-2k; CTK1; CDC2L4; PITALRE; CDC2-related kinase; cell division cycle 2-like protein kinase 4; cell division protein kinase 9; serine/threonine protein kinase PITALRE; tat-associated kinase complex catalytic subunit; cyclin-dependent kinase 9
Gene ID 1025
mRNA Refseq NM_001261.3
Protein Refseq NP_001252.1
UniProt ID P50750

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