Active Recombinant Human CDK7/CCNH protein, His-tagged

• Cat.No.: CDK7-CCNH-313H
• Product Overview: Recombinant full-length human CDK7 and CCNH were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tags.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: CDK7 gene is a member of the cyclin-dependent protein kinase family that is important regulators of cell cycle progression. CDK7 forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). CDK7 is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. CDK7 is thought to serve as a direct link between the regulation of transcription and the cell cycle.
• Form: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
• Bio-activity: The specific activity of CDK7/CyclinH1 was determined to be 56nmol/min/mg as per activity protocol.
• Molecular Mass: CDK7 ~41~43 kDa, and CCNH ~39 kDa
• Purity: The purity of CDK7/CCNH was determined to be >70% by densitometry.
• Applications: Kinase Assay; WB
• Stability: 1 year at -70 centigrade
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL

Gene Information

• Gene Name: cyclin dependent kinase 7 [ Homo sapiens (human) ]; cyclin H [ Homo sapiens (human) ]
• Official Symbol: CDK7; CCNH
• Synonyms: CAK; CAK1; HCAK; MO15; STK1; CDKN7; P39mo15; Cyclin H1; CCNH; CAK; p34; p37
• Gene ID: 1022; 902
• mRNA Refseq: NM_001799; NM_001239
• Protein Refseq: NP_001790.1; NP_001230.1
• MIM: 601955; 601953
• UniProt ID: P50613; P51946