Recombinant Human CDCP1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: CDCP1-058H
• Product Overview: Biotinylated Recombinant Human CDCP1 protein(Q9H5V8-1)(Phe30-Leu666), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His-Avi
• Protein length: Phe30-Leu666
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 74.6 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CDCP1 CUB domain containing protein 1 [ Homo sapiens ]
• Official Symbol: CDCP1
• Synonyms: CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; CD318; SIMA135; membrane glycoprotein gp140; transmembrane and associated with src kinases; subtractive immunization M plus HEp3-associated 135 kDa protein; TRASK
• Gene ID: 64866
• mRNA Refseq: NM_022842
• Protein Refseq: NP_073753
• MIM: 611735
• UniProt ID: Q9H5V8