Recombinant Human CD86 protein, His-tagged
Cat.No. : | CD86-2229H |
Product Overview : | Recombinant Human CD86 protein (Leu26-Pro247), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293). |
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Species : | Human |
Source : | HEK293 |
Tag : | His |
Protein Length : | 222 |
Description : | Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy. |
Form : | Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
Molecular Mass : | The protein has a calculated MW of 26.7 kDa. The protein migrates as 42-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Endotoxin : | Less than 1.0 EU per ug by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 centigrade to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name | CD86 |
Official Symbol | CD86 |
Synonyms | CD86; CD86 molecule; CD28LG2, CD86 antigen (CD28 antigen ligand 2, B7 2 antigen); T-lymphocyte activation antigen CD86; B lymphocyte antigen B7 2; B7 2; B7.2; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); B70; B7-2; LAB72; CD28LG2; MGC34413 |
Gene ID | 942 |
mRNA Refseq | NM_001206924 |
Protein Refseq | NP_001193853 |
MIM | 601020 |
UniProt ID | P42081 |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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