Recombinant Human CD86 protein, His & T7-tagged

• Cat.No.: CD86-7780H
• Product Overview: Recombinant Human CD86 aa. (Gln46~Cys282 (Accession # P42081)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 30.9kDa
• Protein length: Gln46~Cys282
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.2.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: CD86 CD86 molecule [ Homo sapiens (human) ]
• Official Symbol: CD86
• Synonyms: CD86; CD86 molecule; B70; B7-2; B7.2; LAB72; CD28LG2; T-lymphocyte activation antigen CD86; B-lymphocyte activation antigen B7-2; BU63; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; FUN-1
• Gene ID: 942
• mRNA Refseq: NM_001206924.1
• Protein Refseq: NP_001193853.1
• UniProt ID: P42081