Recombinant Human CD52 Protein, hFc-tagged, FITC conjugated

• Cat.No.: CD52-393HF
• Product Overview: FITC conjugated recombinant human CD52 (Gly25-Ser36) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: CAMPATH-1 antigen, also known as cluster of differentiation 52 (CD52), is a glycoprotein that in humans is encoded by the CD52 gene.It is widely expressed on the cell surface of immune cells, such as mature lymphocytes, natural killer cells (NK), eosinophils, neutrophils, monocytes/macrophages, and dendritic cells (DCs).ligation of cell surface CD52 molecules may offer costimulatory signals for T-cell activation and proliferation.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 27.9 kDa. The protein migrates as 38-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Gly25-Ser36
• N-terminal Sequence Analysis: Gly 25
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with FITC via amines
  Excitation source: 488 nm spectral line, argon-ion laser
  Excitation Wavelength: 488 nm
  Emission Wavelength: 535 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD52
• Official Symbol: CD52
• Synonyms: CD52; CD52 molecule; CD52 antigen (CAMPATH 1 antigen) , CDW52; CAMPATH-1 antigen; he5; cambridge pathology 1 antigen; epididymal secretory protein E5; CD52 antigen (CAMPATH-1 antigen); CDW52 antigen (CAMPATH-1 antigen); human epididymis-specific protein 5; CDW52
• Gene ID: 1043
• mRNA Refseq: NM_001803
• Protein Refseq: NP_001794
• MIM: 114280
• UniProt ID: P31358