Recombinant Human CD40 protein, His-Avi-tagged, Biotinylated
| Cat.No. : | CD40-2222H |
| Product Overview : | Biotinylated Recombinant Human CD40 protein(P25942)(Glu21-Arg193), fused with C-terminal His and Avi tag, was expressed in HEK293. |
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| Source : | HEK293 |
| Species : | Human |
| Tag : | C-His-Avi |
| Protein length : | Glu21-Arg193 |
| Conjugation/Label : | Biotin |
| Form : | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Bio-activity : | Immobilized Biotinylated Human CD40, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA. |
| Molecular Mass : | The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
| Endotoxin : | Less than 1EU per μg by the LAL method. |
| Purity : | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Storage : | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Reconstitution : | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Gene Name | CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ] |
| Official Symbol | CD40 |
| Synonyms | CD40; CD40 molecule, TNF receptor superfamily member 5; TNFRSF5, tumor necrosis factor receptor superfamily, member 5; tumor necrosis factor receptor superfamily member 5; Bp50; p50; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily, member 5; nerve growth factor receptor-related B-lymphocyte activation molecule; CDW40; TNFRSF5; MGC9013; |
| Gene ID | 958 |
| mRNA Refseq | NM_001250 |
| Protein Refseq | NP_001241 |
| MIM | 109535 |
| UniProt ID | P25942 |
Not For Human Consumption!
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