Recombinant Human CD28 Protein, Alexa Fluor 555 conjugated

• Cat.No.: CD28-321HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CD28 (P10747-1) (Met 1-Pro 152) was expressed with six amino acids (LEVLFQ) at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: LEVLFQ
• Protein Length: 141
• Form: Lyophilized
• Molecular Mass: The recombinant human CD28 consists of 141 amino acids and predicts a molecular mass of 15.9 kDa. It migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Asn 19
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens ]
• Official Symbol: CD28
• Synonyms: CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290
• Gene ID: 940
• mRNA Refseq: NM_001243077
• Protein Refseq: NP_001230006
• MIM: 186760
• UniProt ID: P10747