Recombinant Human CD276 Protein, hFc-tagged, Alexa Fluor 555 conjugated
Cat.No. : | CD276-303HAF555 |
Product Overview : | Alexa Fluor 555 conjugated recombinant human CD276 (Gly27-Thr461) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293). |
Availability | March 10, 2025 |
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Species : | Human |
Source : | HEK293 |
Tag : | Fc |
Protein Length : | Gly27-Thr461 |
Description : | Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern. |
Form : | Lyophilized |
Molecular Mass : | The protein has a calculated MW of 73.1 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
N-terminal Sequence Analysis : | Gly 27 |
Endotoxin : | < 1.0 EU/ μg by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 555 via amines With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name | CD276 |
Official Symbol | CD276 |
Synonyms | CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3 |
Gene ID | 80381 |
mRNA Refseq | NM_001024736 |
Protein Refseq | NP_001019907 |
MIM | 605715 |
UniProt ID | Q5ZPR3 |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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