Recombinant Human CD248 protein, His-tagged

• Cat.No.: CD248-5033H
• Product Overview: Recombinant Human CD248 protein(Q9HCU0-1)(Gln18-Leu687), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Gln18-Leu687
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 72.7 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD248 CD248 molecule, endosialin [ Homo sapiens ]
• Official Symbol: CD248
• Synonyms: CD248; CD248 molecule, endosialin; CD164 sialomucin like 1 , CD164L1, CD248 antigen, endosialin; endosialin; TEM1; tumor endothelial marker 1; 2610111G01Rik; CD164 sialomucin-like 1; CD248 antigen, endosialin; CD164L1; MGC119478; MGC119479
• Gene ID: 57124
• mRNA Refseq: NM_020404
• Protein Refseq: NP_065137
• MIM: 606064
• UniProt ID: Q9HCU0