Recombinant Human CD24 protein, mFc-tagged, Biotinylated(Primary Amine Labeling)

• Cat.No.: CD24-5743H
• Product Overview: Biotinylated Recombinant Human CD24 protein(P25063-1)(Ser27-Gly59)(Primary Amine Labeling), fused with C-terminal mFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-mFc
• Protein length: Ser27-Gly59
• Conjugation/Label: Biotin; Primary Amine
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-CD24 Antibody at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.31μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD24 CD24 molecule [ Homo sapiens ]
• Official Symbol: CD24
• Synonyms: CD24; CD24 molecule; CD24 antigen (small cell lung carcinoma cluster 4 antigen); signal transducer CD24; CD24A; FLJ22950; FLJ43543; MGC75043
• Gene ID: 100133941
• mRNA Refseq: NM_013230
• Protein Refseq: NP_037362
• MIM: 600074
• UniProt ID: P25063