Recombinant Human CD200R1 protein(Met1-Leu266), His-tagged

• Cat.No.: CD200R1-3202H
• Product Overview: Recombinant Human CD200R1 (AAI43394.1) (Met 1-Leu 266) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: 1-266 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CD200R1 at 1 μg/ml (100 μl/well) can bind human CD200 with a linear range of 0.12-16 ng/ml.
• Molecular Mass: The secreted recombinant human CD200R1 comprises 251 amino acids after removal of the signal peptide and has a predicted molecular mass of 28.2 kDa. As a result of glycosylation, rh CD200R1 migrates as an approximately 50-60 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD200R1 CD200 receptor 1 [ Homo sapiens ]
• Official Symbol: CD200R1
• Synonyms: CD200R1; CD200 receptor 1; MOX2 receptor , MOX2R; cell surface glycoprotein CD200 receptor 1; CD200R; HCRTR2; OX2R; MOX2 receptor; CD200 cell surface glycoprotein receptor; cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; MOX2R
• Gene ID: 131450
• mRNA Refseq: NM_138806
• Protein Refseq: NP_620161
• MIM: 607546
• UniProt ID: Q8TD46

Case Study

Case 1: Hu Y, et al. Tissue Cell. 2024

Diabetic retinopathy is a leading cause of vision issues globally, largely due to oxidative stress, but the role of CD200R protein in this process isn't clear. In this research on human retinal cells, they observed that high glucose levels boosted CD200R expression over time. Reducing CD200R helped decrease oxidative stress and improved cell health, particularly when using ROS scavengers. Researchers also discovered that the AKT/mTOR pathway is involved, as knocking down CD200R affected protein expressions related to this pathway. Blocking mTOR with Rapamycin significantly reduced stress caused by high glucose, highlighting a potential treatment target for diabetic retinopathy.

Fig1. WB of CD200R protein expression.

Fig2. ARPE-19 cells were transfected with an empty vector (EV) or plasmid expressing CD200R.

Case 2: Chen X, et al. J Neuroinflammation. 2023

Neuroinflammation and microglia contribute significantly to depression, with CD200, a protein found in neurons, and its receptor CD200R1, located in microglia, being key players. However, their exact role in depression isn't fully known. In studies using chronic social defeat stress (CSDS) in mice, researchers found that CD200 levels dropped in a brain area related to stress. Boosting CD200 helped reduce signs of depression, while its reduction made mice more stress-sensitive. Moreover, when CD200R1 receptors on microglia were reduced, CD200 couldn't ease depressive symptoms. Microglia were more active after stress exposure, but increasing CD200 calmed them, reduced inflammation, and boosted brain cell growth, helping counteract stress-induced neurogenesis issues.

Fig1. Representative immunostaining for CD200R1 (green)/Iba1 (red)/DAPI (blue) in the DG.

Fig2. Analysis of immobility time in TST of control and CSDS mice injected with LV-siCD200R1 and CD200Fc.

Application

CD200R1, known as CD200 receptor 1, is crucial in how the immune system works. This receptor is mostly on myeloid and T-cells and belongs to the immunoglobulin superfamily. Its role involves partnering with CD200 to bring down inflammation-causing substances like TNF-α and interferon, helping keep inflammation under control. Researchers, especially when working with recombinant CD200R1 proteins, investigate its part in immune response regulation in inflammatory conditions. These studies cover autoimmune diseases, challenges with organ transplants, and how tumors sometimes evade immune defenses. In cancer exploration, scientists also look at how CD200R1 and CD200 interactions might curb anti-tumor responses, examining its potential as a checkpoint target. In terms of practical applications, recombinant CD200R1 is incredibly useful. Scientists use this protein to explore the signal pathways in various cells, such as macrophages and dendritic cells, trying to grasp how it regulates immune responses and interacts with other molecules. Additionally, it's an essential tool for developing specific antibodies used in several laboratory techniques like Western Blot and flow cytometry which help detect CD200R1 presence in tissues. From an industrial angle, producing recombinant CD200R1 involves advanced genetic engineering to express the protein in HEK293 cells, followed by purification processes. These proteins not only aid in crafting diagnostic kits - especially for tests like ELISA - but also hold promise in developing immune-modulating drugs that target the CD200/CD200R1 pathway to potentially enhance anti-tumor responses. To sum up, recombinant CD200R1 proteins are a versatile asset in both research and industrial production. They are pivotal in studies pertaining to immune regulation, inflammation, and tumor immunity, showing a bright future in practical applications, from foundational research tools to advancing diagnostics and therapeutic solutions.

Fig1. CD200:CD200R1 axis. (Katarzyna Kotwica-Mojzych, 2021)

Fig2. CD200-CD200R axis is considered to be a pair of checkpoint molecules that regulate tumor-specific immune responses. (Jin-Qing Liu, 2020)