Active Recombinant Human CD19 protein, Fc-tagged

• Cat.No.: CD19-3307H
• Product Overview: Recombinant Human CD19(Pro 20 - Pro 278) fused with with a human IgG1 with a human IgG1 Fc tag tag at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Pro 20 - Pro 278
• Predicted N Terminal: Pro 20
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse anti-human CD19 Mab FMC63 or HIB19 10 μg/mL ( 100 μl/well ) can bind Human CD19 (20-278), Fc Tag with a linear ranger of 10-1000 ng/mL.Measured by its binding ability in a functional ELISA. Immobilized Human CD9, His Tag at 2 μg/mL (100 μl/well) can bind Human CD19 (20-278), Fc Tag with a linear range of 0.05-0.5 μg/mL.
• Molecular Mass: Has a calculated MW of 55.1 kDa. DTT-reduced Protein migrates as 62-70 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the Human CD19 (20-278), Fc Tag by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Publications:
  Development of a cGMP-compliant process to manufacture donor-derived, CD45RA-depleted memory CD19-CAR T cells (2022)

Gene Information

• Gene Name: CD19 CD19 molecule [ Homo sapiens ]
• Official Symbol: CD19
• Synonyms: CD19; CD19 molecule; CD19 antigen; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4; B4; CVID3; MGC12802
• Gene ID: 930
• mRNA Refseq: NM_001178098
• Protein Refseq: NP_001171569
• MIM: 107265
• UniProt ID: P15391
• Chromosome Location: 16p11.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; BCR signaling pathway, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem
• Function: receptor signaling protein activity