Recombinant Human CD19 Protein (20-291), C-His-tagged

• Cat.No.: CD19-26H
• Product Overview: Recombinant human CD19 (20-291) was expressed in CHO cells using a C-terminal His tag.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 20-291
• Description: B-lymphocyte antigen CD19 is a type I transmembrane protein present on all B cells and is a dominant signaling component of mature B cells. CD19 acts as a critical coreceptor for B cell receptor (BCR) signal transduction by recruiting and amplifying the activation of Src-family protein tyrosine kinases such as Lyn and Fyn. CD19 also enhances BCR-induced signaling crucial for B cell expansion through recruitment and activation of PI3K and downstream AKT kinases. CD19-deficient humans and mice exhibit hypo-responsiveness to transmembrane signals, and weak T cell-dependent humoral responses, leading to an overall impaired immune response.
• Molecular Mass: Observed MW: ~50-65 kDa.
  Calculated MW: ~31 kDa.
• Purity: The purity of CD19 was determined to be ~ 75 % by densitometry.
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.2 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: CD19 CD19 molecule [ Homo sapiens (human) ]
• Official Symbol: CD19
• Synonyms: CD19; CD19 molecule; CD19 antigen; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4; B4; CVID3; MGC12802
• Gene ID: 930
• mRNA Refseq: NM_001178098
• Protein Refseq: NP_001171569
• MIM: 107265
• UniProt ID: P15391