Recombinant Human CD164 protein(Met1-Asp162), His-tagged

• Cat.No.: CD164-3966H
• Product Overview: Recombinant Human CD164 (Q04900-1) extracellular domain (Met 1-Asp 162) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Asp162
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CD164 consists of 150 amino acids and has a predicted molecular mass of 15.9 kDa. As a result of different glycosylation, the apparent molecular mass of rhCD164 is approximately 40-110 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD164 CD164 molecule, sialomucin [ Homo sapiens ]
• Official Symbol: CD164
• Synonyms: CD164; CD164 molecule, sialomucin; CD164 antigen, sialomucin; sialomucin core protein 24; MGC 24; MUC 24; MGC-24v; multi-glycosylated core protein 24; MGC-24; MUC-24; endolyn
• Gene ID: 8763
• mRNA Refseq: NM_001142401
• Protein Refseq: NP_001135873
• MIM: 603356
• UniProt ID: Q04900

Case Study

Case 1: Zhang XG, et al. Cancer Med. 2018

This study explored CD164 in bladder cancer, finding it linked to worse outcomes like metastasis. Using immunohistochemistry and other methods, researchers showed that silencing CD164 reduced tumor growth and spread by influencing key proteins. CD164 also often pairs with CXCR4 in tumors, making it a potential target for bladder cancer treatment.

Fig1. The expression of proliferation related proteins PCNA was down-regulated by silencing of CD164.

Fig2. The expression of CD164 in mice tumors was dramatically decreased by Western blot.

Case 2: Zhang XW, et al. Oncol Rep. 2020

Colon cancer is a leading cause of cancer deaths. This research highlights that the lncRNA RP11-619L19.2 is highly expressed in colon cancer, linked to advanced stages and metastasis. Silencing it reduces tumor growth, migration, and EMT, by interacting with miR-1271-5p and CD164. This mechanism of RP11-619L19.2 offers new potential targets for colon cancer treatment.

Fig1. The relative expression of CD164 in HCT-116 or SW620 cells transfected with miR-1271-5p mimics or miR-NC was evaluated by RT-qPCR analysis.

Fig2. The relative expression levels of CD164 protein were evaluated by western blot analyses.

Application

CD164, also known as Endolyn or MUC-24, is a membrane glycoprotein found in various tissues like bone marrow and lymph nodes. It plays a key role in cell adhesion, signaling, and the movement and proliferation of hematopoietic stem cells. In research, recombinant CD164 protein is crucial for studying its involvement in processes like cell migration and adhesion, impacting things like muscle formation and stem cell homing. It's particularly useful in understanding hematopoiesis, as it regulates how precursor cells stick and grow, and in exploring cancer biology, given its abnormal expression in cancers like prostate and colorectal cancer. In industrial applications, recombinant CD164 is produced using gene engineering in mammalian cells like HEK293 and purified for use. It's a valuable tool for developing diagnostic assays that target CD164, such as those used in ELISA tests. This protein also holds promise in drug development, where it's used to create fusion proteins that combine with growth factors to improve drug stability. Such innovation could have therapeutic applications in treating tumors and diseases affecting blood formation. Overall, recombinant CD164 protein is essential in both research and industrial production. Its role in cell adhesion, hematopoietic regulation, and tumor biology highlights its importance, making it a significant tool for advancing our understanding and treatment of various diseases.

Fig1. Defining hCD164 epitopes by glycosidase treatments. (Suzanne M Watt, 2021)