Recombinant Human CD14 protein, His-tagged

• Cat.No.: CD14-789H
• Product Overview: Recombinant Human CD14 aa. (Asp125~Leu288 (Accession # P08571)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Asp125~Leu288
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: CD14 CD14 molecule [ Homo sapiens (human) ]
• Official Symbol: CD14
• Synonyms: myeloid cell-specific leucine-rich glycoprotein; monocyte differentiation antigen CD14
• Gene ID: 929
• mRNA Refseq: NM_000591.3
• Protein Refseq: NP_000582.1
• UniProt ID: P08571