Recombinant Human CCL5 protein, His,SUMO-tagged

• Cat.No.: CCL5-3533H
• Product Overview: Recombinant Human CCL5 protein(P13501)(Ser24-Ser91), fused with N-terminal His,SUMO tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&SUMO
• Protein Length: Ser24-Ser91
• Tag: N-His,SUMO
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tris,0.3M NaCl (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CCL5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 21 kDa. Due to the protein can cleaved into two chains, so it migrates to 15-18 kDa and 22-25 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CCL5 chemokine (C-C motif) ligand 5 [ Homo sapiens ]
• Official Symbol: CCL5
• Synonyms: CCL5; chemokine (C-C motif) ligand 5; D17S136E, SCYA5, small inducible cytokine A5 (RANTES); C-C motif chemokine 5; beta chemokine RANTES; MGC17164; RANTES; regulated upon activation; normally T expressed; and presumably secreted; SIS delta; SISd; small inducible cytokine subfamily A (Cys Cys); member 5; T cell specific protein p288; T cell specific RANTES protein; TCP228; eoCP; SIS-delta; beta-chemokine RANTES; small-inducible cytokine A5; T-cell specific protein p288; t cell-specific protein P228; T-cell-specific protein RANTES; eosinophil chemotactic cytokine; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; D17S136E
• Gene ID: 6352
• mRNA Refseq: NM_002985
• Protein Refseq: NP_002976
• MIM: 187011
• UniProt ID: P13501