Recombinant Human CASTOR1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CASTOR1-3060H
• Product Overview: GATSL3 MS Standard C13 and N15-labeled recombinant protein (NP_001032755) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Functions as an intracellular arginine sensor within the amino acid-sensing branch of the TORC1 signaling pathway. As a homodimer or a heterodimer with CASTOR2, binds and inhibits the GATOR subcomplex GATOR2 and thereby mTORC1. Binding of arginine to CASTOR1 allosterically disrupts the interaction of CASTOR1-containing dimers with GATOR2 which can in turn activate mTORC1 and the TORC1 signaling pathway.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 36.3 kDa
• AA Sequence: MELHILEHRVRVLSVARPGLWLYTHPLIKLLFLPRRSRCKFFSLTETPEDYTLMVDEEGFKELPPSEFLQVAEATWLVLNVSSHSGAAVQAAGVTKIARSVIAPLAEHHVSVLMLSTYQTDFILVREQDLSVVIHTLAQEFDIYREVGGEPVPVTRDDSSNGFPRTQHGPSPTVHPIQSPQNRFCVLTLDPETLPAIATTLIDVLFYSHSTPKEAASSSPEPSSITFFAFSLIEGYISIVMDAETQKKFPSDLLLTSSSGELWRMVRIGGQPLGFDECGIVAQIAGPLAAADISAYYISTFNFDHALVPEDGIGSVIEVLQRRQEGLASTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CASTOR1 cytosolic arginine sensor for mTORC1 subunit 1 [ Homo sapiens (human) ]
• Official Symbol: CASTOR1
• Synonyms: CASTOR1; cytosolic arginine sensor for mTORC1 subunit 1; GATSL3; cytosolic arginine sensor for mTORC1 subunit 1; GATS protein like 3; GATS-like protein 3; cellular arginine sensor for mTORC1 protein 1
• Gene ID: 652968
• mRNA Refseq: NM_001037666
• Protein Refseq: NP_001032755
• MIM: 617034
• UniProt ID: Q8WTX7