Recombinant Human Caspase 2, Apoptosis-related Cysteine Peptidase

• Cat.No.: CASP2-747H
• Product Overview: Recombinant human caspase-2 was expressed inE. colias a single polypeptide chain. MW = 12000 and 19000.

Specification

• Cat. No.: CASP2-747H
• Description: Caspase 2 proteolytically cleaves other proteins. It belongs to a family of cysteine proteases called caspases that only cleave proteins at an amino acid following a aspartic acid residue. Within this family, caspase 2 is part of the Ich-1 subfamily. It is one of the most conserved caspases in different species of animal. Caspase 2 has a similar amino acid sequence to initiator caspases, including caspase 1, caspase 4, caspase 5 and caspase 9. It is produced as a zymogen, which contains a long pro-domain that is similar to that of caspase 9 and contains a protein interaction domain known as a CARD domain. Pro-caspase-2 contains two subunits, p19 and p12.
• Source: E.coli.
• Purity: ≥90% by SDS-PAGE.
• Form: Lyophilized solid.
• Recommended Reaction Conditions: 50 mM HEPES, pH 7.2, 50 mM NaCl, 0.1% CHAPS (Cat. No. 220201), 10 mM EDTA, 5% glycerol, 10 mM DTT, and 200 µM peptide substrate. For analyzing caspase-2 activity, use 1 unit of enzyme per assay.
• Specific Activity: ≥1500 units/mg protein.
• Unit Definition: One unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-pNA per h at 37°C, pH 7.2.
• Solubility: PBS (0.1-1 unit/µl).
• Toxicity: Standard Handling (MSDS available upon request).
• Storage: ≤ -70℃. Following reconstitution, aliquot and freeze (-70℃). Stock solutions are stable for up to 6 months at -70℃.
• Tag: Non

Gene Information

• Gene Name: CASP2 caspase 2, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Synonyms: CASP2; caspase 2, apoptosis-related cysteine peptidase; ICH1; NEDD2; CASP-2; ICH-1L; ICH-1L/1S; caspase-2; NEDD-2; ICH-1 protease; protease ICH-1; NEDD2 apoptosis regulatory; neural precursor cell expressed, developmentally down-regulated 2; neural precursor cell expressed developmentally down-regulated protein 2; EC 3.4.22.55; Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12
• Gene ID: 835
• mRNA Refseq: NM_032982
• Protein Refseq: NP_116764
• MIM: 600639
• UniProt ID: P42575
• Chromosome Location: 7q34-q35
• Pathway: Signalling by NGF
• Function: cysteine-type endopeptidase activity; enzyme binding; identical protein binding; peptidase activity; protein binding; protein domain specific binding