Recombinant Human CASP1

• Cat.No.: CASP1-596H

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.
• Sourse/Host: E. coli.
• Formulation: Lyophilized.
• Reconstitution: Reconstitute to 1U/µl with PBS containing 15% glycerol.
• Specific Activity: ~20'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-pNA (pNA:p-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
• Application: Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
• Storage: For long term storage, -80°C.
• Pathways: Amyotrophic lateral sclerosis (ALS); Cytosolic DNA-sensing pathway; NOD-like receptor signaling pathway

Gene Information

• Gene Name: CASP1 caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) [ Homo sapiens ]
• Synonyms: caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); ICE; P45; IL1BC; CASP1; caspase-1; CASP-1; IL-1BC; IL1B-convertase; CASP1 nirs variant 1; IL-1 beta-converting enzyme; interleukin 1-beta convertase; interleukin-1 beta convertase; interleukin 1-B converting enzyme; interleukin-1 beta-converting enzyme; caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase); p45; EC 3.4.22.36
• Gene ID: 834
• mRNA Refseq: NM_001223
• Protein Refseq: NP_001214
• MIM: 147678
• UniProt ID: P29466
• Chromosome Location: 11q23
• Function: cysteine-type endopeptidase activity; caspase activator activity; peptidase activity; protein binding