Recombinant Human carbonic anhydrase III, muscle specific, His-tagged

• Cat.No.: CA3-35H
• Product Overview: Recombinant Human Carbonic anhydrase III produced inE.Coli, and having a molecular mass of 33.9 kDa. CAIII is expressed with an amino-terminal hexahistidine tag. The CA-III is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Carbonic anhydrase (carbonate dehydratase) is a family of metalloenzymes (enzymes that contain one or more metal atoms as a functional component of the enzyme) that catalyze the rapid (and reversible) conversion of carbon dioxide to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. Carbonic anhydrase greatly increases the rate of the reaction, with typical catalytic rates of the different forms of this enzyme ranging between 104 and 106 reactions per second. The active site of most carbonic anhydrases contains a zinc ion. CAIII is a cytoplasmic isoenzyme, but is released into the circulation following injury.
• Physical Appearance: Sterile Filtered blue solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Supplied in 1x Laemmli Buffer: 25 mM Tris-HCl pH6.8; 50 mM DTT; 1% SDS; 0.1% Bromophenol Blue and 2.5% Glycerol.
• Stability: CAIII although stable at 14°C for 1 week; should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Publications:
  Local pH Gradient Initiated by Light on TiO2 for Light-Triggered Modulation of Polyhistidine-Tagged Proteins (2016)

Gene Information

• Gene Name: CA3 carbonic anhydrase III, muscle specific [ Homo sapiens ]
• Synonyms: CA3; carbonic anhydrase III, muscle specific; Car3; CAIII;Carbonic anhydrase 3;CA-III; Carbonate dehydratase III; Carbonic anhydrase III; carbonic anhydrase III, muscle specific; EC 4.2.1.1
• Gene ID: 761
• mRNA Refseq: NM_005181
• Protein Refseq: NP_005172
• UniProt ID: P07451
• Chromosome Location: 8q13-q22
• MIM: 114750
• Pathway: Nitrogen metabolism
• Function: carbonate dehydratase activity; lyase activity; metal ion binding; nickel ion binding; zinc ion binding