Recombinant Human Calcium/Calmodulin-Dependent Protein Kinase I, His-tagged

• Cat.No.: CAMK1-5372H
• Product Overview: Recombinant humanfull length CAMK1 with His tag is expressed in insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: CAMK1 is an enzyme that in humans is encoded by theCAMK1 gene. CAMK1 is expressed in many tissuesand is a component of a calmodulin-dependent protein kinase cascade.Calcium/calmodulin directly activates CAMK1 by binding to the enzyme andindirectly promotes the phosphorylation and synergistic activation of theenzyme by CAMK1 kinase.
• Form: Liquid in50 mM Tris, pH 7.5 + 150 mM NaCl + 0.5 mM EDTA + 0.02% Triton X-100 + 2 mMDTT + 50% glycerol.
• Molecular Weight: 45.4 kDa
• Concentration: 0.36 mg/ml
• Purity: ≥90% as determined by SDS-PAGE analysis.
• Specific Activity: 1353nmole of phosphate transferred to Autocamtide-2 peptide substrate(KKALRRQETVDAL) per minute per mg of total protein at 30°C. Activitydetermined at a final protein concentration of 0.83 μg/ml.
• Storage: Stable for 6 monthsin working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: CAMK1
• Full Length: Full L.

Gene Information

• Gene Name: CAMK1calcium/calmodulin-dependent protein kinase I [ Homo sapiens ]
• Synonyms: CAMK1;calcium/calmodulin-dependent protein kinase I; CAMKI; MGC120317; MGC120318;calcium/calmodulin-dependent protein kinase type 1; caM-KI; caMKI-alpha;OTTHUMP00000158527; caM kinase I alpha; EC 2.7.11.17; CaM kinase I
• Gene ID: 8536
• mRNA Refseq: NM_003656
• Protein Refseq: NP_003647
• MIM: 604998
• UniProt ID: Q14012
• Chromosome Location: 3p25.3
• Pathway: Calcium Regulation in theCardiac Cell
• Function: ATP binding; calmodulinbinding; calmodulin-dependent protein kinase activity; nucleotide binding