Recombinant Human C4BPA protein, His-tagged

• Cat.No.: C4BPA-49H
• Product Overview: Recombinant Human C4BPA(Gln548~Leu597) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Gln548~Leu597
• Description: This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Two pseudogenes of this gene are also found in the cluster.
• Form: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 7.1kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB. If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Publications:
  Fetal lung C4BPA induces p100 processing in human placenta (2019)

Gene Information

• Gene Name: C4BPA complement component 4 binding protein, alpha [ Homo sapiens ]
• Official Symbol: C4BPA
• Synonyms: C4BPA; complement component 4 binding protein, alpha; C4BP, complement component 4 binding protein, alpha; C4b-binding protein alpha chain; proline-rich protein; PRP; C4BP
• Gene ID: 722
• mRNA Refseq: NM_000715
• Protein Refseq: NP_000706
• MIM: 120830
• UniProt ID: P04003
• Chromosome Location: 1q32
• Pathway: CD40/CD40L signaling, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Pertussis, organism-specific biosystem
• Function: protein binding