Recombinant Human c-ERBB2

• Cat.No.: ERBB2-18H
• Product Overview: Recombinant Human c-erbB2 encoding 537-636 amino acids expressed inE.colic-erbB2 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 537-636 a.a.
• Description: c-erbB-2 is a receptor tyrosine kinase of the c-erbB family. It is closely related instructure to the epidermal growth factor receptor. c-erbB-2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE.
• Formulation: c-erbB2 at 100 µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Application And Suggested Dilutions: • ELISA • Inhibition Assays • Western Blotting.
• Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 37 kDa.
• Storage: Store vial at -20ºC to -80ºC. When stored at the recommended temperature, this protein is stable for 12 months. Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
• Synonyms: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); NEU; NGL; HER2; TKR1; CD340; HER-2; HER-2/neu; ERBB2; erbB-2; herstatin; c-erb B2/neu protein; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); C-erbB-2; EC 2.7.10.1; p185erbB2; CD340 antigen ; MLN 19; NEU proto-oncogene; Tyrosine kinase-type cell surface receptor HER2
• Gene ID: 2064
• mRNA Refseq: NM_001005862
• Protein Refseq: NP_001005862
• MIM: 164870
• UniProt ID: P04626
• Chromosome Location: 17q11.2-q12; 17q21.1
• Pathway: Adherens junction; Bladder cancer; Calcium signaling pathway; Endometrial cancer; ErbB signaling pathway; Focal adhesion; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Axon guidance
• Function: ATP binding; ErbB-3 class receptor binding; Hsp90 protein binding; epidermal growth factor receptor activity; glycoprotein binding; growth factor binding; identical protein binding; nucleotide binding; protein C-terminus binding; protein heterodimerization activity; protein phosphatase binding; receptor signaling protein tyrosine kinase activity; transferase activity; transmembrane receptor activity; ubiquitin protein ligase binding

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.