Recombinant Human BTK, GST-tagged

• Cat.No.: BTK-530H
• Product Overview: Recombinant full-length human BTK was expressed by baculovirus inSf9 insect cellsusing a N-terminal GST tag. MW=106kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation. BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors. Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. The unique role of BTK makes it a desirable target for potential anti-cancer, anti-inflammatory and anti-viral agents as well as other treatments.
• Purity: >80%.
• Specific Activity: 23 pmol/min/μg.
• Assay condition: The enzyme reaction wascarried out for 1h at room temperature in abuffer containing 50 mM HEPES (pH7.5),10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 μM of ATP. Substrate : 2 μM Tyr peptide 1 from Invitrogen.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: BTK Bruton agammaglobulinemia tyrosine kinase [ Homo sapiens ]
• Synonyms: BTK; Bruton agammaglobulinemia tyrosine kinase; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1; MGC126261; MGC126262; OTTHUMP00000023676; OTTHUMP00000063593; Bruton tyrosine kinase; B-cell progenitor kinase; tyrosine-protein kinase BTK; agammaglobulinemia tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase
• Gene ID: 695
• mRNA Refseq: NM_000061
• Protein Refseq: NP_000052
• MIM: 300300
• UniProt ID: Q06187
• Chromosome Location: Xq21.33-q22
• Pathway: B cell receptor signaling pathway; Fc epsilon RI signaling pathway
• Function: ATP binding; identical protein binding; kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein tyrosine kinase activity; transferase activity; zinc ion binding