Recombinant Human Bruton Agammaglobulinemia Tyrosine Kinase, His-tagged

• Cat.No.: BTK-995H
• Product Overview: Recombinant full-length human BTK was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation. BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors. Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. The unique role of BTK makes it a desirable target for potential anti-cancer, anti-inflammatory and anti-viral agents as well as other treatments.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 78 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25 % glycerol.
• Specific Activity: 43 nmol/min/mg
• Purity: > 90 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: B Cell Receptor Signaling Pathway; B cell receptor signaling pathway; BCR signaling pathway; Class I PI3K signaling events; EPO signaling pathway; FAS (CD95) signaling pathway; Fc epsilon RI signaling pathway; Fc-epsilon receptor I signaling in mast cells; IL-5 Signaling Pathway; IL-6 Signaling Pathway; Kit Receptor Signaling Pathway; Osteoclast differentiation; Primary immunodeficiency

Gene Information

• Gene Name: BTK Bruton agammaglobulinemia tyrosine kinase [ Homo sapiens ]
• Official Symbol: BTK
• Synonyms: BTK; Bruton agammaglobulinemia tyrosine kinase; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1; MGC126261; MGC126262; tyrosine-protein kinase BTK; OTTHUMP00000023677; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase; Tyrosine-protein kinase BTK; Agammaglobulinaemia tyrosine kinase; EC 2.7.10.2; OTTHUMP00000023676
• Gene ID: 695
• mRNA Refseq: NM_000061
• Protein Refseq: NP_000052
• MIM: 300300
• UniProt ID: Q06187
• Chromosome Location: Xq21.33-q22
• Function: ATP binding; identical protein binding; kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphatidylinositol-3,4,5-trisphosphate binding; protein binding; protein kinase activity; protein tyrosine kinase activity; transferase activity; zinc ion binding