Recombinant Human BRCC3, His-tagged

• Cat.No.: BRCC3-10280H
• Product Overview: Recombinant Human BRCC3 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 14-316a.a.
• Description: This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: BRCC3 BRCA1/BRCA2-containing complex, subunit 3 [ Homo sapiens ]
• Official Symbol: BRCC3
• Synonyms: BRCC3; BRCA1/BRCA2-containing complex, subunit 3; chromosome X open reading frame 53 , CXorf53; lys-63-specific deubiquitinase BRCC36; BRCC36; C6.1A; BRISC complex subunit BRCC36; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 36; CXorf53; RP11-143H17.2
• Gene ID: 79184
• mRNA Refseq: NM_001018055
• Protein Refseq: NP_001018065
• MIM: 300617
• UniProt ID: P46736
• Chromosome Location: Xq28
• Function: enzyme regulator activity; metal ion binding; metallopeptidase activity; peptidase activity; polyubiquitin binding; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity